Dear Mohammad Afeef

do you mean DSF (thermofluor) for thermal shift assay?

If, yes, using which DSF approach? The sypro orange based? or the label free (nanotemper)?

Since the buffer composition may alter the thermal stability of your protein in theory is it better to do it in the buffer that you use for the storage of the protein to have more reilable data.

Unfortunatelly if you are yuo using the sypro orange this is not ever possibile since in some cases the buffer may bind the probe and provide positive signals (e.g if it contain detergents) but you can simply test it by perfroming a blank experiment using only buffer in place of the protein sample.

good luck

Manuele