For the co-sedimentation assays, does anyone know how to choose the rpm for particular molecular weight of protein?

Jijumon A.S. @Jijumon-As

24 October 2015 0 4K Report

For the co-sedimentation assays, can anyone knows how to choose the rpm for particular molecular weight of protein?

Badges
Science topic

More Jijumon A.S.'s questions See All

How I can find a validated questionnaire to measure "house money effect" in financial market (Likert scale)?

Is any validated questionnaire available to measure "House money effect" bias in the financial market (Likert scale)?

11 July 2024 6,772 4 View

Can we realtime stream data from Vicon Nexus (Streaming Vicon Blue Trident IMU data) to LabView?

We are using Vicon Blue trident IMUs for motion analysis. Nexus is used to stream data from IMUs currently, which are giving accelerometer, gyro, magnetometer and global angles. These data are...

04 July 2024 1,130 0 View

How do you look for scopus indexed journals with a high acceptance rate and shorter peer review process?

I am a PhD scholar looking for a Q1,Q2 Scopus indexed journal related to the domain of adolescent mental health and stigma, preferably with a good acceptance rate and quicker publication process....

07 February 2024 8,808 2 View

How can I train and test CNN ?

Hello everone. I'm very new in ML and DL models, and i want to use CNN to train and test it on my dataset, i had very large dataset and it is already split into 80% train and 20% test. I wrote...

06 February 2024 1,250 2 View

What happens in this double slit experiment?

The arrangement is shown in the figure in the attachenment. One of the slit is having a glass slab in front of it. if photon is assumed as wave, then the part of it moving in the lower slit...

03 December 2023 5,349 1 View

MATLAB or MATHEMATICA code for solving the system of ODE of higher order by any numerical method?

I am trying to solve the system in the following figure by any numerical method. Can you please suggest me a source to learn this with Mathematica or Matlab?

02 August 2023 3,435 3 View

How to Determine an best solution from the Pareto solution set?

In some cases, it is difficult for a decision-maker to express an explicit approximation of the preference function. Therefore, it can be effective to allow the decision-maker to choose from a...

17 May 2023 7,484 0 View

Could anyone give me some help validating my interview schedule for my research?

Hi, this is Manjari here. I am currently pursuing a Ph.D. at Christ University. My research is titled “Development and Testing the Efficacy of a Mental Health Awareness and Destigmatisation (MHAD)...

24 August 2021 9,191 5 View

Can anyone suggest a scale to measure stigma levels among adolescents in the Indian context?

My name is Manjari; I am pursuing my Ph.D. at Christ University. I am conducting research titled "Development and Testing the Efficacy of a Mental Health Awareness and Destigmatisation (MHAD)...

05 May 2021 1,870 3 View

How to implement monte carlo method to compute state value in matlab ?

assume there are 80 states each with a possibility of 2 actions. we have a predefined reward function. The problem is about research allocation.

30 October 2020 10,169 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View