I isolated soil bacteria, from that i want to test siderophore production, many papers showing different protocols, which is the best and easiest protocol for testing sideropore production. here iam giving one easiest protocol collected from google is it useful? suggest one easy and best protocol.

CAS agar Ingredients g/l Chrome Azurol S 60.5mg/50ml d.w. Hexadecyltrimethyl ammonium bromide 72.9mg/40ml d.w. Kings Medium B base 42.23 Distilled water 900ml pH 6.8±0.2 *Mix Chrome Azurol S solution and Hexadecyltrimethyl ammonium bromide solution and then add it to 10 ml of 1mM FeCl3.6H2O solution prepared in 10 mM HCl. Add the final solution to 900 ml of Kings Medium B base.

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