My Aim is to make THP-1 cell line GFP+. Which methods are the best to get fused expressable GFP or maybe just GFP. I have three options
1. Used lentivrus but i am not sure about its efficiency in THP-1 and which lentirus plasmid maybe the best option.
2. I should use piggyBac but then again i don't have plamsid for transposes and its very expensive if bought from company.
3. I should directly do the transfection with expressable vectors
Add an antibiotic selection cassette. Do you have other transposon systems around the freezers in your lab? Have you asked other nearby labs if they have the plasmids? If you have plasmids for Cas9 expression you could try integration by homologous recombination.
Hanif Ullah
Use the lentiviral vector system; it works really well. Don't worry about the efficiency; you can select based on antibiotics available with the plasmid. Just be careful about starting antibiotic selection from lower concentrations and increase it with every passage.
Pscalps Puromycin or hygromycin can be requested from addgene. Please let me know if you need more details.
All the best.
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