Hanif Ullah

4 Questions 12 Answers 0 Followers

Questions related from Hanif Ullah

For making stable THP1 cell lin of GFP, which method can be used? Or In other words if some can explain in simple words how can i make GFP + cell lin?

My Aim is to make THP-1 cell line GFP+. Which methods are the best to get fused expressable GFP or maybe just GFP. I have three options 1. Used lentivrus but i am not sure about its efficiency in...

18 March 2023 7,614 3 View

Does anyone have experience in infecting suspension cells (like THP-1 or K562 etc) by retrovirus?

I am trying to make stably expressing cell line of my gene of interest in Suspension cell line using retrovirus but i don't have recommended protocol. I have searched literature but mostly they...

15 December 2020 8,421 3 View

I need protocol for making cell line immortalized, if anyone have please let me know or at least guide me?

I have sorted cell line but the problem is that it proliferate well upto three or four passages after that their growth slows down making me wait for long time. So i am thinking to make it...

27 April 2019 9,666 4 View

How can i over express the plasmid in THP-1 cells? Which method is the most suitable to get the high efficiency?

Before i did with Lentivirus infection but the cells die, i don't the reason. Also the plasmid size is little long its about 15kb. If anyone have any idea how to get the overexpression of Plasmid...

01 December 2017 3,570 1 View