Hanif Ullah

5 Questions 14 Answers 0 Followers

Questions related from Hanif Ullah

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

20 July 2024 130 6 View

For making stable THP1 cell lin of GFP, which method can be used? Or In other words if some can explain in simple words how can i make GFP + cell lin?

My Aim is to make THP-1 cell line GFP+. Which methods are the best to get fused expressable GFP or maybe just GFP. I have three options 1. Used lentivrus but i am not sure about its efficiency in...

18 March 2023 7,629 3 View

Does anyone have experience in infecting suspension cells (like THP-1 or K562 etc) by retrovirus?

I am trying to make stably expressing cell line of my gene of interest in Suspension cell line using retrovirus but i don't have recommended protocol. I have searched literature but mostly they...

15 December 2020 8,450 3 View

I need protocol for making cell line immortalized, if anyone have please let me know or at least guide me?

I have sorted cell line but the problem is that it proliferate well upto three or four passages after that their growth slows down making me wait for long time. So i am thinking to make it...

27 April 2019 9,678 4 View

How can i over express the plasmid in THP-1 cells? Which method is the most suitable to get the high efficiency?

Before i did with Lentivirus infection but the cells die, i don't the reason. Also the plasmid size is little long its about 15kb. If anyone have any idea how to get the overexpression of Plasmid...

01 December 2017 3,580 1 View