How deep learning with IoT can be applied in agriculture?suggest any specific application of agriculture ?

Application of agriculture

01 January 2019 9,107 3 View

Can any one suggest me alternate substrate for alpha amylase (PPA) assay?

20 March 2017 5,761 3 View

What is the reason for peak splitting in XRD? Can reitveld refinement be done for the same?

18 November 2016 8,730 16 View

Can we consider negative result of sample in antioxidant assay?

In all antioxidant assay for calculation below mentioned formula is used % of Inhibition = CONTROL O.D - Sample O.D/ CONTROL O.D X 100 But, In this case I got negative result of sample because...

10 August 2015 9,438 15 View

Which control should be used for my calculation: a positive which has standard compound or a negative control which has reagents only

19 May 2015 3,845 2 View

How do you plot a tafel plot?

12 January 2015 1,498 6 View

How to synthesize graphene from graphite?

I want to synthesise graphene from graphite. Can anyone give me an easy procedure for that?

26 February 2014 1,914 10 View

Plasmid DNA isolation for shRNA vectors giving very low yield with Stbl4 transformation

My vector in which I cloned shRNA primers have a very high copy. I cloned shRNA plasmids and then tried to transform them in Stbl4 but I'm getting very very low or almost negligible concentration...

07 April 2013 3,819 15 View

Whats the best way to transfect Hela Cells? We are using PEI but efficiency is very low.

We are using Hela cells for checking localization of proteins by Immunofluorescence and transfecting these cells with PEI, but efficiency is just 30 %. How to improve it? Should we use another...

24 September 2012 2,606 16 View

Double In situ Hybridization

I need to do Double In situ Hybridization , is anyone having optimized protocol for it , Please help me with this , we have been doing In situ hybridization in our lab using DIG labelled probe...

20 April 2012 2,083 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View