I am an undergraduate student planning for my undergraduate thesis. That being said, I would love to learn more on isolation studies or analysis. To expound my question, the analysis that I will be conducting are NMR and LC-MS. I would like to know how much of the isolate do I need to have the best results in my characterization study. Another question would be on how to have an optimal isolation technique. My technique right now is using Reverse Phase - Solid Phase Extaction for further fractionation and then a preparative run of HPLC to isolate the compound of interest. Do you have any tips or recommendation to optimize my yield so that I can accomplish my undergraduate thesis on an earlier time.
Hello, see attached info may be useful for you!
Good luck!
for NMR max 50 mg is required, in case of 1H NMR it needed lesser than as required in 13C NMR. for LC-MS microgram of samples are sufficient, UV-Vis also very less amount of sample required, FT-IR depending upon the type of method its quantity is varied over all 30 mg will be sufficient. In case of TLC for confirmation of Rf value same more amount like 1.00 gm sample is required, to establish the solvent system for particular compound. for checking M.P. some more quantity of samples are required as depend upon type of instrument (digital of manual).
I would just like to give my gratitude to Eshak Mourad El-Hadidy , Karen A. Darbinyan , Sadiq S Mareai , Zafar Iqbal , Punit R Bhatt
, and M. Jameel for your contributions or answers that you have provided. I must say, the journals were very useful for my study. Hope you all have a wonderful 2019- Badges
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