For cultivation you could use any rich medium such as Nutrient agar (NB), for identification first you need to know what species you are looking for? then you need to search previous works (literature) for how to identify it, if you have access to molecular biology facility then use it for identification.
Hope this helps
You can use Trypton Soy agar (TSA) after that you need to isolate each genus as sing colony for identification or you can use multiplex PCR for identification , I recommend you to read more in previous works.
you can use nutrient agar or tryptone glucose yeast extract agar media as universal media, then determine gram staining to determine if it gram positive or negative , then follow Bergys manual to identify isolates biochemically, then molecular identification was done using 16 sRNA was done to estimate , I recommended you to read more for isolation and identification of isolates.
If you are downwind from a sewage plant use EMB agar for isolation of gram negative rods. If you are down wind from wind blowing across a barn yard use Mannitol salt agar to isolate Micrococci and Staphylococci.
Hi Parvaiz,
It will depend on what you are trying to find. The techniques used in compost bio-aerosols monitoring are containing in the attached methods, these are a good starting point.
The culturable fraction of the population is only a small part if you are looking for total viable and other particulates then a laser method with staining or real time PCR.
Andrew
Both articles listed above should be read and studied by the investigator. I should have mentioned that we used a 6 stage Andersen sampler.
To follow on from previous message. The new methods with Europe for waste facilities have been revised in the UK by the EA in M9, these are based on German methods.
The national physical laboratory in the UK have done some work on standarisation
I agree with Andrew as you should consider bioaerosols monitoring techniques that may be suitable for specific identification and enumeration. Otherwise, depending on the kind of bacteria you want to select, you can consider selective media.
In addition to general media such as Nutrient Agar or Tryptic Soy Agar you might use selective chromogenic agars to narrow down the organismic diversity to the bacteria of interest. There is a series of these agars available (see attachment). We used them successfully for the detection of Bacillus species.
using general media as nutrient agar or TGY media , then choose the same colonies in morphological characterization then, using gram stain to differentiate between Gram positive and Gram negative bacteria ,then using biochemical identification according to Berg,s manual
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