For Cacodylate-citrate buffer, how much of the following needed (g/L): 0.01 M sodium cacodylate, 0.45 M sodium chloride and 0.10 M trisodium citrate?

12 December 2017 0 10K Report

Dear ResearchGate, to make the buffer (pH 7) for measuring phenoloxidase activity (immunology) of shrimp, please. Thank you.

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Can we sample hepatopancreas of shrimp, keep at 4oC, perform Total cultivable bacteria (TSA) and Presumptive Vibrio (TCBS) counts after a week?

Dear ResearchGate, does anyone know please? Thank you.

03 April 2018 1,783 0 View

Feed additives in marine shirmp: is it more useful to conduct Immunological, Electron Microscopy analyses following growth trial or disease challenge?

Dear ResearchGate, to determine mode of action (if only one choice), please. Thank you.

10 November 2017 5,714 0 View

May we have descriptions on the landscape, habitat and physical environment of Manggis River and Sungai Papar (Kampung Kogopon, Papar, Sabah), please?

Dear ResearchGate, this info is for Anguilla eel samples caught from the stream/river. Thank you.

11 December 2016 6,879 4 View

Is it possible to have 8 replicates for the control and 4 for each treatment, please? Can ANOVA be performed on treatments with different replicates?

Dear ResearchGate, this question is for an aquaculture nutrition study. Has anyone come across this experimental design, please? Thank you.

01 January 1970 4,240 0 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View