For Cacodylate-citrate buffer, how much of the following needed (g/L): 0.01 M sodium cacodylate, 0.45 M sodium chloride and 0.10 M trisodium citrate?

12 December 2017 0 10K Report

Dear ResearchGate, to make the buffer (pH 7) for measuring phenoloxidase activity (immunology) of shrimp, please. Thank you.

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More Kok Onn Kwong's questions See All

Can we sample hepatopancreas of shrimp, keep at 4oC, perform Total cultivable bacteria (TSA) and Presumptive Vibrio (TCBS) counts after a week?

Dear ResearchGate, does anyone know please? Thank you.

03 April 2018 1,843 0 View

Feed additives in marine shirmp: is it more useful to conduct Immunological, Electron Microscopy analyses following growth trial or disease challenge?

Dear ResearchGate, to determine mode of action (if only one choice), please. Thank you.

10 November 2017 5,807 0 View

May we have descriptions on the landscape, habitat and physical environment of Manggis River and Sungai Papar (Kampung Kogopon, Papar, Sabah), please?

Dear ResearchGate, this info is for Anguilla eel samples caught from the stream/river. Thank you.

11 December 2016 6,943 4 View

Is it possible to have 8 replicates for the control and 4 for each treatment, please? Can ANOVA be performed on treatments with different replicates?

Dear ResearchGate, this question is for an aquaculture nutrition study. Has anyone come across this experimental design, please? Thank you.

01 January 1970 4,300 0 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

How do I prepare a working stock solution of 14C sodium bicarbonate to get a final activity of 3uCi?

I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...

15 July 2024 5,898 0 View