It says in the manual that for metabolomic analyses, we should use biological replicates instead of the regular replicates. I am just concerned if even though i already have 3 biological replicates, do I still need test or analyze each biological replicate in certain number of replicates too? For example I have 3 biological replicates, if I test each biological replicates in triplicates again, I have a total of 9 replications ?
Or are those biological replicates good to go and I can just get the mean as is?
Dear Duri Eun,
The following information may be helpful:
https://www.researchgate.net/post/Triplicate-for-samples-in-qPCR-analysis
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3321166/
https://www.licor.com/bio/blog/technical-and-biological-replicates
https://totalinternalreflectionblog.com/2017/09/06/seeing-triple-a-short-guide-to-experiment-reproducibility/
https://www.illumina.com/Documents/products/technotes/technote_power_replicates.pdf
Hi Dr Duri Eun . See the following link: https://www.researchgate.net/post/When-is-meaningful-to-exclude-biological-replicates-from-further-qPCR-analysis
See the following link: https://www.ndsu.edu/faculty/horsley/ExptSize.pdf
Also see the following link: https://www.researchgate.net/post/How_can_I_know_how_many_replicates_repetition_and_reproducibility_is_required_for_my_microbiology_work_Is_there_any_formula_for_that
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