Global Warming effects?

Hi Professionals! Due to Global warming the earth temperature increases (https://climate.nasa.gov/effects/), which generates floods, storms, and tornadoes because of melting of ice glacier, in...

08 February 2021 9,038 8 View

Hello I have a question about solar cells (perovskite solar cells) modeling in COMSOL?

I have done all optical simulations in RF module, now how can i find generation rate of electrons and holes, secondly how can i use these generation rate in semiconductor module to find further...

11 December 2020 6,652 3 View

Expertise in Photoelectrochemical spectroscopy (PES) and Voltammetry Techniques?

Collaboration Opportunity: Please send me a personal message, if you have expertise in the following tools, with specific applications to Corrosion Research. 1: In-Situ Photoelectrochemcial...

06 January 2020 7,468 1 View

How to Maximize Protein Extraction Yield from Avibacterium?

Dear all, I'm currently working with Avibacterium (close family with Haemophillus). I want to compare the expression of an outer membrane protein across different growth phases. There's no MAb...

02 January 2019 3,420 3 View

For writing and organizing data related to Systematic review and meta-analysis, what can be the other soft tools besides Revman 5?

Software's for systematic review and meta-analysis other than Revman?

08 June 2017 5,989 2 View

How much the requirement of percent recovery for validation method analysis sample from natural product?

Read 4 answers by scientists to the question asked by Bachtiar Ihsan on Jun 2, 2016

01 June 2016 2,221 3 View

Does over-use trypsin have an effect on digestion for proteomics , if it does , what type of impact does it have on protein expression?

in FASP (Filter assisted sample preperation) prosedure, the enzyme to protein ratio needed for digestion of a protein sample is 1:100, if it is used over -use trypsin, what kind of problem can be...

22 May 2016 3,624 9 View

What is the stability of frozen tissues used for protein expression?

18 January 2016 5,510 2 View

Has anyone ever achieved a click reaction in acetic acid or acetic acid-organic solvent mixture ?

Altough I already know that click reaction is not pH sensitive still wonder if any reasearch with click reaction in acetic acid solution was made or not.

08 October 2015 4,106 1 View

How should I work with the aluminum isopropoxide so I can avoid the possible harms?

I am planning to use the aluminium isopropoxide as a ring opening polymerization catalyst. I know it is a highly flammable solid so I am going to set the reaction at the room temperature. Are...

03 February 2015 4,498 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View