Any body doing MI in mice and staining heart slices with tetrazolium at @37°C?
Most often I am facing problem, heart slices distort / contract in tetrazolium incubation @37°C. Staining itself has no problem, but the tissue structure.
Briefly:
Can any body suggest the remedy for this.
Note: This does not matter whether MI was performed or not.
Hello Ihsan,
I have done TTC staining for multiple studies with similar success. Here is the paper, you might have read already using interesting guidance: "The sections are then removed from the vial, and excess moisture is blotted; a possible distortion can be mended by gently flattening the sections between the index finger and the thumb. Here is the PMID 19820193
Please read carefully whole method section whether you perform it similar way, there might be still some guidance for you.
Hope this helps,
Filip
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