Has anyone used Histopaque 1077 to separate live and dead culture cells?

We need to separate dead cells out from our neuroblastoma cell line, and continue culturing live ones. Sigma website mentions histopaque 1077 reagent can be used for this purpose (also used to...

09 October 2013 487 9 View

Troubleshooting insert amplification from a plasmid: Has anyone done TAIL-PCR using a plasmid template?

I have been trying to amplify a puromycin insert from a plasmid from Addgene. I have tried Hot-Start reaction, Pfu turbo polymerase- the later has been a little bit better. Still, not getting...

05 June 2013 3,045 6 View

Need to do a PCR reaction with long primers (60-100 nt) - looking for protocol

Amplicon lengths are 800bp - 1.8kb. Primers are longer than 'usual' - are in 60nt - 100nt range. Looking for advice on Dos/Donts for such a reaction. Primer pair for insert 1: F:...

05 June 2013 880 11 View

Experiencing problems sequencing plasmid after cloning: see sharp peaks at the beginning; signal

Trying to sequence pCDNA vector after cloning in multiple inserts. Sequencing primers align

05 June 2013 8,366 23 View

>20deg differential between Tm values of F- & R- primers - how to set annealing temperature for PCR?

F primer has Tm around 70deg, while R-primer's Tm is 43deg. I am planning on doing a gradient PCR- not sure what is the lowest value to set for annealing, without risking non-specific...

05 June 2013 3,596 7 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Which is better medicine or food in obesity?

Preference in terms of the scientific aspect

28 February 2021 796 2 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

What is the most prevalent drug related problem in the developed countries?

Detecting the drug related problems is an important step in the pharmaceutical care plan

21 February 2021 5,421 3 View

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19 February 2021 8,647 3 View