Reeteka Sud

6 Questions 24 Answers 0 Followers

Questions related from Reeteka Sud

Has anyone used Histopaque 1077 to separate live and dead culture cells?

We need to separate dead cells out from our neuroblastoma cell line, and continue culturing live ones. Sigma website mentions histopaque 1077 reagent can be used for this purpose (also used to...

10 October 2013 609 9 View

>20deg differential between Tm values of F- & R- primers - how to set annealing temperature for PCR?

F primer has Tm around 70deg, while R-primer's Tm is 43deg. I am planning on doing a gradient PCR- not sure what is the lowest value to set for annealing, without risking non-specific...

06 June 2013 3,654 7 View

Need to do a PCR reaction with long primers (60-100 nt) - looking for protocol

Amplicon lengths are 800bp - 1.8kb. Primers are longer than 'usual' - are in 60nt - 100nt range. Looking for advice on Dos/Donts for such a reaction. Primer pair for insert 1: F:...

06 June 2013 1,001 11 View

Troubleshooting insert amplification from a plasmid: Has anyone done TAIL-PCR using a plasmid template?

I have been trying to amplify a puromycin insert from a plasmid from Addgene. I have tried Hot-Start reaction, Pfu turbo polymerase- the later has been a little bit better. Still, not getting...

06 June 2013 3,104 6 View

Experiencing problems sequencing plasmid after cloning: see sharp peaks at the beginning; signal

Trying to sequence pCDNA vector after cloning in multiple inserts. Sequencing primers align

06 June 2013 8,412 23 View

Follow-up questions

"EVALUATION OF VYAGHRI HARITAKI IN MANAGEMENT OF KASA-SHWASA... Dear authors, 1. How was the selected dose decided upon? 2. In the text, you mention lab tests being done to assess adverse...

01 January 1970 6,293 0 View