Any suggestions on a good fluro dye one can use, to label Myelin with, to study internalization of myelin into cells??
FluoroMyelin Red, Neutral Red etc.
I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...
22 February 2021 1,400 4 View
I obtained a XRD diffractogram with significant broad band at low 2theta levels, also around 30 2theta another smaller broad band appears. It all points out to the existence of an amorphous phase....
19 February 2021 8,414 4 View
Does anyone know how to measure the complexity of an artificial neural network? Could you provide me references? Thanks a lot!
25 January 2021 2,710 10 View
In the paper A wavelet-based method for power-line interference removal i... we propose a new method for removing PLI. This method consists in identifying the ECG and noise frequency range for...
29 December 2020 9,233 3 View
Hi! I would like to know what tools I could use to analyze interactions between a new lncRNA and possible protein/miRNA targets. The biggest problem I had to find such tools is that the RNA we...
20 November 2020 8,331 3 View
I am trying to isolate an allkylated imidazole from a mixture containing unreacted 1H-imidazole. The problem is that imidazole is slightly soluble in my targed molecule. Furthermore, I need to...
04 November 2020 4,513 3 View
Hello! I'm working on a systematic review (without meta-analysis) and when evaluating the quality of the papers only one presented low quality. I did not find guidelines in the instrument itself...
04 November 2020 6,564 7 View
Microdata are a wonderful way of analyzing specific issues in an heterogeneous environment. Brazil is wonderfully diverse country, and its countryside is also extremely heterogeneous. We have...
25 October 2020 8,900 2 View
Greetings Everyone. I am reading several articles about biochars and notice several use N2 atmosphere inside the muffle furnace. can you produce biochars without this N2 atmosphere or other inert...
17 August 2020 8,992 6 View
Dear all, i am depositing pure Al on a DC Magnetron sputtering tool and I am getting melted targets all the time. The target dimensions are: diameter 50mm and thickness 4mm. A bit of historical...
22 June 2020 3,780 1 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I run algorithms like HDBSCAN and MeanShift, and compare them with K-means and GaussianMixture. The first two algorithms do not return cluster labels for some elements in the dataset. My question...
27 February 2021 428 3 View
Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...
26 February 2021 9,119 3 View
I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...
23 February 2021 6,756 1 View
a free multi label clinical text dataset , containing text as entry and several labels as classes. Best regards
22 February 2021 5,988 2 View
New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...
21 February 2021 3,278 3 View
Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...
19 February 2021 472 5 View
I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...
18 February 2021 4,392 4 View
Hello, it is about creating a dataset for semantic segmentation with three classes. The problem is that one class dominates with >90% and one class is
18 February 2021 4,254 5 View