Hello everybody,
My boss wants me to do the following experiment:
we have mice infected with BCG i.v for 3 days. then spleens were collected and frozen in tissue-tek.
Now I have to do fluorescent IHC staining for Phospho-Syk, Phospho-Fyn, Phospho-Erk1/2 to detect T cells activation, co-stained with CD4/CD8.
Is it possible to see these kinase activation in frozen tissue?
Does anyone has any experience with such staining?
Thanks
Rahul Kumar
Yes it is possible if you use suitable antibody. We routinely use IHC in frozen tissue sections.
0 votes
0 thanks
Kellie Bowen
Make sure you don't use PBS when looking at Phosphorated proteins. TBS!
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Biological Science
- Anatomy
- Tissue
More Salina Begum's questions See All
Similar questions and discussions