Salina Begum

4 Questions 4 Answers 0 Followers

Questions related from Salina Begum

Fluorescent IHC staining for Phospho-Syk, Phospho-Fyn, Phospho-Erk1/2 in frozen sections of murine spleen ?

Hello everybody, My boss wants me to do the following experiment: we have mice infected with BCG i.v for 3 days. then spleens were collected and frozen in tissue-tek. Now I have to do...

02 February 2018 6,836 2 View

Is there a way to re-stimulate antigen specific Th1 cells with anti-CD3/anti-CD28?

Hello everyone,I have established an antigen specific Th1 cell line. I have already re-stimulated them 2 times with irradiated APCs and appropriated antigen.Now, I am looking for a easier method...

08 August 2016 5,395 3 View

How can I get rid of granulocytes form my CD4+ cell culture?

Hi,I have been co-culturing antigen specific CD4+ cells with T cell depleted irradiated splenocytes as antigen presenting cells. after 6 days I can see my culture is full of granulocytes and very...

07 July 2016 4,915 3 View

How do I dissolve my peptide in a sterile way?

hello,I have two peptides one is 11 AA and another is 15 AA long.I got them delivered in powdered form.Now I want to dissolve it. One is soluble in water and another is in DMSO.But the problem is...

01 January 2016 6,967 5 View