What are the major flavonoids in the tomatoes? Naringenin, rutin, quercetin, kaempferol, calconaringerin or myrecitin?
Some of the flavone glycosides have weird solubilities. I look at narirutin, naringin, hesperidin and didymin in citrus and citrus juices via HPLC/UPLC-dad 280 nm and it is necessary to have 40% DMSO in the injection solvent.
HPLC-PDA can detect naringenin and rutin also. Detection wavelength will be 320nm.
Try this:
Any C18–5mm column. Elution was performed using water-H3PO4 (99.5:0.5) as solvent A and acetonitrile as
solvent B at a flow rate of 1.5 ml/min.
Gud luck!!!
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