Hi, I need a protocol to extract and identify microbiota from fish gut. Any suggestions? Thank you. (P.s. Something low tech as I am technologically limited, guess no funds and big high tech equipment will be available for non profitable topics)
Emil - if you are looking to study the complete microbiome, you need to have access to a lab that can do both the extraction and the analysis (such as NextGen sequencing). These can be expensive, but there are labs which do this for a fairly reasonable price.
If you feel the question you want to answer can be answered by more traditional cultural methods, I can suggest ideas or even try to give you means to get to the questions you are interested in pursuing.