Why should skim-milk be used for blocking buffer in western blots?

I have just performed my first western blot and none of my targeted proteins were detected. The only thing visible on the membrane was the prestained ladder. Someone suggested that my blocking...

21 August 2018 5,787 4 View

How to grid downscale CMIP5 climate data grid to 0.5 degree using Matlab ?

08 July 2018 2,235 2 View

Merging many to 1 in SPSS version 25?

I have 2 datasets one is the adult in the houshold and the other is the child(ren). I'm trying to merge the child data to the adult data however there may be more than 1 child to an adult (many...

10 May 2018 4,946 6 View

Why would LB-low salt medium be prefereable to normal LB medium?

I've come across a protocol that says to use low salt LB broth (10g/L tryptone, 5g/L NaCl, 5g/L yeast extract) for bacterial growth curves. Why would it be preferable to use a low salt medium...

18 February 2018 2,048 2 View

Why is the deletion of bcsQ using lambda red recombination unsuccessful?

I have been unsuccessful in knocking out bcsQ in Pectobacterium carotovorum ssp. brasiliense using a three-step PCR method. Plasmids used: pKD4 and pKD46 bcsQ is MinD homologue part of the...

17 July 2016 1,533 2 View

Has anyone seen this strange FSC-A versus FSC-H pattern?

I recently ran an experiment where I divided a mouse tumor into several wells of a 96-well plate. Each well was stained with a different panel of antibodies. In only one of the wells, I got the...

22 February 2016 4,653 6 View

Does anyone have suggestions about cell density, DIV and surface preparation for hippocampal neuron culturing for micro electrode array?

27 November 2014 4,362 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View