Does sgRNA need Cas9 for targeting?

We know that in the CRISPR Cas9 system, sgRNA and cas9 together cleave/silence/block/bind areas of interest in the genome. However, would sgRNA alone (without the cas9) be able to Target the gene...

08 September 2016 5,385 3 View

Does DAPI stain a Virus?

Hi There were some experiments going on in our lab and I am comparatively new to the field. Cells infected with virus were stained with DAPI. I read about and DAPI and found that they tag to DNA...

10 November 2015 5,379 8 View

What is the intital DNA Library length in SELEX process?

I am planning on developing/selecting a strong binding aptamer for a Viral protein. I found that DNA libraries are available from multiple companies that offer N20, N30, N40 all the way to N200...

08 September 2015 9,987 5 View

Solving Poissons Equation - Why am I unable to get the expected waveform?

The issue is I am able to solve the equations but the output waveforms are not as expected. So mostly I think it is issue with boundary conditions. I feel the boundary conditions I have considered...

06 July 2015 5,247 3 View

Diclofenac Sodium in PEG

I need to add the diclofenac sodium drug into nanoparticles of polyethylene glycol. I don't have access to polymerization techniques so I cannot make PEG from EG. I do not have co- or block...

02 March 2013 5,544 0 View

How to control nanoparticle size while heating?

I am currently trying to make multiple samples of magnetic Nickel Gadolinium ferrites with varying amounts of Gadolinium. The recrystallization (magnetic) temperature of these particles is close...

11 December 2012 8,318 46 View

Difference between a 200 KV and 300 KV TEM

What are the differences in the functionality, resolution and type of samples that are used in a 200 kV and 300 KV TEM? I would like to know the technical differences, what are the exact change...

03 April 2012 1,780 15 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View