Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Schrodinger Desmond runs fine on one GPU, but crashes on other GPU?

Hi, We are having issues using Desmond on our workstation. Simulations run fine on one of our gpus ("Gpu0") but crash consistently on the other gpu ("gpu1" - which is also set up as the display...

01 February 2021 5,866 3 View

How does climate change affect birds (shorebirds)?

I m recently investigating the impact of climate change on shorebird population trends, I would like to know what is the impact of fluctuations in variables such as sea surface temperature, global...

10 November 2020 9,293 15 View

Can I use the EPIC-Oxford FFQ for free?

Designing my PhD project and need to use an FFQ, but we don't have any funding for this part of the project.

09 November 2020 4,541 1 View

Is there a survey tool (similar to REDCap) through which we can send an anonymous survey and restrict respondents to one survey each?

I am working on a research survey where I need to send it out to a group of users and track in (anonymous) responses. I need to restrict users to completing the survey only once. I do not think...

06 August 2020 918 2 View

Western Blot Transfer Band Smearing?

Anyone have any insight? It's becoming a recurring issue. I make fresh transfer buffer each time.

29 July 2020 9,331 9 View

How many users can i put in the same vsat broadband plan?

sorry if i wasn't able to explain very well for example, i am in a country that doesn't have satellite internet service, and would like to be the first provider for this service. lets say i have a...

05 July 2020 4,637 1 View

Failed gateway cloning?

Hi everyone, I am having a few doubts about gateway cloning. We are using gateway cloning for protein expression using pDEST15 as our destination vector. I attached a picture of the pDEST15...

10 March 2020 7,472 2 View

Inrush control when Transformers are Energized at Solar Renewable is best achieved how?

Increasing use of solar renewable resources or farms on weak remote locations are causing significant power quality and voltage notch issues with connecting utilities. High penetration of these...

17 August 2019 7,097 2 View

Glass microballoons from sodium silicate solution?

I once produced microballoons from sodium silicate solution (water glass). does anyone have experience in manufacturing these, are articles you could point me in the direction of? thanks

01 July 2019 8,764 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

How to arrange coordinates and extract climate dataset out of MPI-ESM-MR netcdf files?

Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...

28 February 2021 912 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View