Faint IC bands - why?

Katarzyna Kalinowska @Katarzyna_Kalinowska5

01 January 2019 0 1K Report

Hi Guys,

recently, I have some problems with IC bands.

I do my PCR on clinical samples, I check PCR products for different fungi in 2 separate reactions. IC bands in 1st PCR reaction always look good, meanwhile in 2nd reaction they are faint.

I do the 1st and 2nd electrophoresis in the same buffer (TAE), but I don't think it might be a problem - the same situation occured, when I changed the order (so I did electrophoresis after 2nd PCR reaction first and after that I did electrophoresis of 1st PCR reactions).

Can you help me?

Besides IC bands, everytihing looks OK.

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Science method

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