FACS vs Immunopanning for astrcytes?

12 December 2019 0 4K Report

Hi can anyone tell me the difference and similarity between using FACs or Immunopanning to isolate rodents astrocytes or any other cells?

Badges
Science topic

More Abhishek Dahal's questions See All

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

I have synthesized a composite of ZnO and In2O3 with variations in ZnO content. In Raman , what is the peak observed at 130 cm-1?

Dear all, I have noticed, as the ZnO content increases, the Raman intensity at 100cm-1 also increases. However, there is a peak nearby at 130 cm-1, which I could not find in any paper related to...

28 July 2024 5,410 1 View

What are the trending topic to writ e a review article on it?

The topic should relate to pharmacy ,drug, methos of drug discovery, or any other topic but related to dug or pharmaceutical field

02 July 2024 4,031 3 View

Why my mesh independent fastener as beam cartesian type not working in abaqus?

i have attached my model and i have used point based mesh independent fastener using attachment points and type of connector is basic cartesian but this is not working as i apply displacement on...

02 July 2024 6,293 0 View

How does the foilar application of Banana Pseudostem sap influences the growth as well as yield attributes of oilseed crops?

Also, explain its pros and cons concerning other organic sources of nutrients.

01 July 2024 612 0 View

How to simulate FSS with patch antenna in HFSS ?

I want to simulate square loop fss with antenna so that it can work as a filter . how to simulate it in HFSS

29 June 2024 4,840 1 View

The value of RF3(Z) in Abaqus model is huge how to control that?

I am modelling CFS Sheath wall in abaqus and i am applying the 100 mm displacement but i am getting high reach force close to 150 kN but i need it till 50kN. I have used mesh independent fastner...

27 June 2024 4,212 2 View

Can AI platforms aid in efficient and early prediction of biomarkers w.r.t. haematological malignancies ? If yes, than which AI can be used?

I'm really struggling to find a manuscript on this topic. However, I'm pretty sure about the benefits of using TensorFlow AI tool when it comes to ML, but prediction of Biomarkers is still not...

17 June 2024 3,968 3 View

Is there any relation between UV absorbance and PL emission spectrum?

Hello Everyone, I have done UV-Vis absorbance and PL emission of 3 samples. When plotted, there is a clear correlation between UV-Vis absorption and PL emission spectrums, where the sample with...

06 June 2024 8,937 2 View

How to compute molar Gibbs free energy (chemical potential) for single molecule using Gaussian 09 or 16 software?

I want to calculate the Gibbs free energy of single gas molecule (AsH3) using Gaussian 09, but I am confused between "Thermal correction to Gibbs Free Energy" and "Sum of electronic and thermal...

02 June 2024 6,365 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View