Dear Shifa,

As read in the RNAlater protocol from ThermoScientific:

"Retrieve tissue from RNAlater® Solution with sterile forceps, quickly blot away excess RNAlater® Solution with an absorbent lab wipe or paper towel, and then submerge the sample in RNA isolation lysis solution. Homogenize tissue promptly after placing it in lysis/denaturation solution."

After this you should be able to start isolating RNA using an RNA isolation kit from TS.

Maurice

Thanks..

Which company kit is suitable for tissues stored in RNAlater?

Since i tried with Trizol i got concentration of 198ng/ul

You could proceed with the protocol from Thermo Scientific.

In our lab we use a very nice and easy protocol of the TRIzol method, combined with RNA purification with a kit from Macherey-Nagel to remove genomic DNA.

Thanks a lot.. will try..