Shifa Javed

4 Questions 13 Answers 0 Followers

Questions related from Shifa Javed

Transfection_large plasmid_HEK cells?

Does anyone have any experience with transfecting 12Kb plasmid in HEK cell line using jet prime?

11 November 2019 2,708 3 View

Extraction of RNA from tissue?

Hi. I have brain tissue stored in RNAlater which will be the best method to extract RNA from it.

10 October 2017 2,453 4 View

Why do the western blots look like this?

This is a blot for B actin..

09 September 2017 4,514 3 View

Hi Everyone. i wanted to ask about extraction of proteins from cell lines for SDS PAGE and further western blotting?

I take the cells trypsinize the cells and wash them with cold PBS two times. Then i use RIPA lysis buffer according to the pellet and add protease inhibitors 1/20th volume of lysis buffer. Pipet...

03 March 2017 8,657 3 View