Extraction of Heme A, Heme B and Heme O?

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What is the biological importance of alkaline phosphatase in milk?

Greetings all As you might know raw milk has an alkaline phosphatase activity, and indeed this activity is used for pasteurization quality control. The question I am wondering about is, what is...

05 June 2019 4,596 1 View

Plasmid for constitutive expression of gfp in Bacillus subtilis?

Greetings Does anyone have , and is willing to sent me, a plasmid suitable for constitutive expression of gfp in Bacillus subtilis? Many thanks Yoram

03 April 2019 1,628 1 View

Illuminating a sample with 2 wavelength simulteneously?

Greetings I am looking for an optical setup that will enable me to illuminate a single sample with two different wavelength in the UV range simultaneously. I though about placing 2 monochromators...

10 November 2018 1,686 0 View

What are the carbon 'species' in traeted wastewater? A good model compound for such?

Greetings all As wastewater get biologically treated carbon is removed, but obviously not all carbon species are removed, since some are more easily consumed by the bacteria doing the treatment....

10 November 2018 6,388 2 View

Origin of 220nm absorbance peak of free amino acids?

Greetings I am trying to find out what is the origin of the 220nm absorbance peak of free amino acids? It is usually attributed to peptide bond but I find it also in single aa... Yoram

05 June 2018 6,797 3 View

Why do particles concentrated in water droplet edge?

Greeting. I have noticed a pattern and wonder what is the reason. When ever I have suspension of particles in water droplet, be it bacteria or abiotic they seem to concentrate in the edge of the...

04 May 2018 10,187 1 View

Can I connect HP1047A RI detector to Agilent 1200 HPLC system?

Greetings We need to buy an RI detector for our Agilent 1200 HPLC system (connected to a computer running ChemStation). Given the high price of new detector I thought about buying a used one....

09 October 2017 7,320 10 View

Fluorescent dye that bind to dsRNA but not ssRNA?

Greetings all. We are looking into following the kinetics of short dsRNA melting and about using a dye that will bind differently to dsRNA and ssRNA. Any idea for such a dye? Or to another simple...

06 July 2017 6,823 1 View

Quantifying UV irradiation of different lamps by UV sensor?

Greetings I am trying to quantify UV irradiation form different UV lamps buy using UV sensor. The problem is the response curve of the sensor is different for different wavelength (as it is often...

05 June 2017 7,507 4 View

A simple procedure for coating polymer sheets with photocatalytic TiO2?

Greetings Does anyone have a simple protocol for coating polymer sheets with TiO2 to obtain effective photocatalytic sheets? Transparency is NOT and issue but flexibility will be. A collaboration...

31 December 2016 2,543 2 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View