Extraction of Heme A, Heme B and Heme O?

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What is the biological importance of alkaline phosphatase in milk?

Greetings all As you might know raw milk has an alkaline phosphatase activity, and indeed this activity is used for pasteurization quality control. The question I am wondering about is, what is...

05 June 2019 4,434 1 View

Plasmid for constitutive expression of gfp in Bacillus subtilis?

Greetings Does anyone have , and is willing to sent me, a plasmid suitable for constitutive expression of gfp in Bacillus subtilis? Many thanks Yoram

03 April 2019 1,471 1 View

Illuminating a sample with 2 wavelength simulteneously?

Greetings I am looking for an optical setup that will enable me to illuminate a single sample with two different wavelength in the UV range simultaneously. I though about placing 2 monochromators...

10 November 2018 1,529 0 View

What are the carbon 'species' in traeted wastewater? A good model compound for such?

Greetings all As wastewater get biologically treated carbon is removed, but obviously not all carbon species are removed, since some are more easily consumed by the bacteria doing the treatment....

10 November 2018 6,225 2 View

Origin of 220nm absorbance peak of free amino acids?

Greetings I am trying to find out what is the origin of the 220nm absorbance peak of free amino acids? It is usually attributed to peptide bond but I find it also in single aa... Yoram

05 June 2018 6,591 3 View

Why do particles concentrated in water droplet edge?

Greeting. I have noticed a pattern and wonder what is the reason. When ever I have suspension of particles in water droplet, be it bacteria or abiotic they seem to concentrate in the edge of the...

04 May 2018 10,029 1 View

Can I connect HP1047A RI detector to Agilent 1200 HPLC system?

Greetings We need to buy an RI detector for our Agilent 1200 HPLC system (connected to a computer running ChemStation). Given the high price of new detector I thought about buying a used one....

09 October 2017 7,137 10 View

Fluorescent dye that bind to dsRNA but not ssRNA?

Greetings all. We are looking into following the kinetics of short dsRNA melting and about using a dye that will bind differently to dsRNA and ssRNA. Any idea for such a dye? Or to another simple...

06 July 2017 6,650 1 View

Quantifying UV irradiation of different lamps by UV sensor?

Greetings I am trying to quantify UV irradiation form different UV lamps buy using UV sensor. The problem is the response curve of the sensor is different for different wavelength (as it is often...

05 June 2017 7,322 4 View

A simple procedure for coating polymer sheets with photocatalytic TiO2?

Greetings Does anyone have a simple protocol for coating polymer sheets with TiO2 to obtain effective photocatalytic sheets? Transparency is NOT and issue but flexibility will be. A collaboration...

31 December 2016 2,394 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View