What is the best way extract protein from Pichia pastoris in SDS-PAGE?
Dear Karim,
I enclosed a Manual of Methods for Expression of Recombinant Proteins in Pichia pastoris file, you can find all key points for manipulating Pichia.
Good luck
Thank you.
No, I work with Phil-S1 vector.
This vector doesn't have a His Tag for extraction protein.
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