What is the best way extract protein from Pichia pastoris in SDS-PAGE?
Dear Karim,
I enclosed a Manual of Methods for Expression of Recombinant Proteins in Pichia pastoris file, you can find all key points for manipulating Pichia.
Good luck
Thank you.
No, I work with Phil-S1 vector.
This vector doesn't have a His Tag for extraction protein.
Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...
25 February 2021 9,602 4 View
Dear all, If steel jacketing is used to retrofit a reinforced-concrete (RC) column, can we use it to improve flexural strength? I understand that we leave gaps between the ends of the jacket and...
22 February 2021 1,906 5 View
Hi all, Does SeismoStruct support modeling rectangular RC columns retrofitted with steel jackets (e.g. rectangular, elliptical) ? Any suggestions for other software (apart from...
20 February 2021 4,521 1 View
15 February 2021 1,997 6 View
Hello, I currently have my T4 virus in HEPES buffer. Unfortunately, there are always crystals that are contaminating my solution (due to the buffer in which the Viruses are) when I observe them...
01 February 2021 400 3 View
We want to explore a mediating relationship employing SEM with Amos SPSS. Our independent variable is attitude toward wife abuse. mediator is exposure to male marital violence, and outcome...
07 January 2021 4,481 3 View
I'm using 3 different light intensities (constant values 15,30,45 W) for the wastewater treatment of an organic compound and optimizing the parameters (pH, catalyst dose and light intensity)...
31 December 2020 2,377 4 View
I am looking for a block in MATLAB that can simulate a varying load such as a load of a house given below. this block will be connected to a power system
27 December 2020 5,835 5 View
I study and research about anxiety disorders in children in Iran and I sometimes face questions like this and unfortunately I could not find article or book to answer that.
30 October 2020 6,822 7 View
30 October 2020 961 3 View
How to calculate the RMSD values for a MD simulation using MOE?
07 August 2021 0 0 View
When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.
16 June 2021 0 0 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...
03 March 2021 5,978 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View