Does anyone know any manufacturers or suppliers that are providing sucrose gradient ultracentrifugation? Most manufacturers seem to provide density gradient ultracentrifugation so any help or insight would be beneficial?
We can't help you with a solution based on sucrose gradient ultracentrifugation, but we are performing quite good results using our SEC columns for isolating exosomes.
In particular, when we want to isolate them from a complex sample (i.e. plasma), we use our SEC columns and then, if we want to concentrate them, we use Amicon100K.