I am confused about inhibitor expression concentration when calculating IC50 of the alfa-glucosidase assay. Should inhibitor concentration be expressed as initial or as final concentration?
It should be the concentration that is present during the enzyme reaction.
I have conduct alpha glucosidase inhibitory assay. But i'm confuse why the absorbance of the sample was highest than blank (without sample), as well as using acarbose as positive control. Anyone...
24 March 2023 1,154 0 View
How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.
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I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
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There are three factors involved in the activity, the substrate acting as a substrate, the substrate acting as an inhibitor and another inhibitor for which you want to measure the Ki.
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Hello esteemed scientists, I am endeavoring to increase the yield of my recombinant protein. It is a 120 kDa protein tagged with MBP and features three intrinsically disordered regions with pI...
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I had done calculating MTT assay using Slope intercept method - Excel Four parameter logistic regression model-web portal Using Graphpad prism. Among them which one is the best and suitable for...
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I have purchased Acetylcholiesterase from Electrophus electricus (electric eel), C3389-2KU, following details are written on the enzyme vial. Type-VI S lyophilized powder 200-1000 Units/mg...
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Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...
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I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...
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I know that conventional wisdom dictates that protease inhibitors should be added to lysis buffers when extracting protein to prevent sample degradation. That said, why are such inhibitors...
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