I performed DLS on purified exosomes with an EXO SPIN kit (precipitation and size exclusion chromatography) the Z-average was too big and the result wasn’t good I should mention that to break up aggregations I do sonication before analysis. Does anyone have any idea? Could filtering through 0.2 µm be a solver?
If you are interested in nanosizes, yes you should filter the sample. Sonication only helps with agglomerations, not aggregations.
Hi,
Exosome size measurements through DLS tend to be variable , thus should be repeated several times in single as well as multiple different preparations, in order to reach a cumulative particle size range . Please pay attention towards QC report at the end of each measurement ('Good' report means acceptable results). Further you may need to modify the default DLS protocol method in order to fit EV measurements.
Filtering through 0.2um could be helpful but sonication will break up the EV particles giving incorrect sizes.
NTA is a better method to quantify EV size and concentration.
Regards
Neha
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