Fatemeh Norouzi

5 Questions 2 Answers 0 Followers

Questions related from Fatemeh Norouzi

Dose "sodium perborate" in the TMB protocol refer to sodium perborate monohydrate or sodium perborate tetrahydrate?

Hi I will make a TMB solution but I am confused with one of the reagents. in protocols doesn't mention the type of sodium perborate (monohydrate or tetrahydrate) can you help me with the correct...

08 January 2025 3,536 2 View

what is the effect of in vitro transcribed RNA secondary structure and thermodynamic energy in translation?

Hi everyone, I am going to design a template for in vitro transcription. I add the T7 promoter sequence (taatacgactcactatag) before the target template sequence and insert two other Gs after the...

21 April 2022 9,583 0 View

Does anyone have experience with intramuscular injection of exosomes?

Hi all, I want to know what is the fate of exosomes after intramuscular injection. is there any report about the distribution rate of exosomes between muscular and blood cells after an...

08 October 2021 7,443 3 View

Exosome preparation for DLS (dynamic light scattering)

I performed DLS on purified exosomes with an EXO SPIN kit (precipitation and size exclusion chromatography) the Z-average was too big and the result wasn’t good I should mention that to break up...

01 January 1970 2,613 2 View

Autophagy gating in flow cytometry

Hi all, I use flow cytometry to assess autophagy in my cells through acridine orange staining. I am facing a challenge in gating to determine the red/green ratio. I'm unsure how to draw the gate,...

01 January 1970 3,435 0 View