I am hoping to optimise my exosomes yield when ultracentrifuging conditioned media of cultured cells.

Protocol so far is:

30 min at 2,000x g (remove cells and large debris)

60 min at 10,000 x g (remove apoptotic bodies and larger microvesicles)

120 min at 100,000 x g (pellet exosomes)

This seems to hover around the standard protocol used (minus PBS wash, filtration, sucrose gradient etc.) however my question is about the consumables involved.

Does anybody know if any specific plastic tube for UC has lower binding affinity of exosomes (surface proteins or lipids)? And likewise if better yield is achieved when storing in low protein-binding eppendorfs (e.g. LoBind)?

Any experience/comments welcome!

Thank you,

Sam