Exist any method to quantify cytokines and chemokines in mouse brain tissue using any RNA multiplex assays?

More Fabian Lombillo's questions See All

Cross-country research: How can I control for the factor country, although I don't have any hypotheses regarding country differences?

Hello, I am currently preparing a preregistered report for experiments in different countries. I am studying motivations/values of students; therefore, I want to conduct several same experiments...

28 January 2021 5,763 3 View

How best can one separate n-hexane-soluble fraction of plant extract that binds strongly to silica gel columns and what are the possible compounds?

I wanted to elute n-hexane-soluble fraction of a plant extract in a silica gel column using different proportions of dichloromethane/n-hexane. The bands separated visibly initially but moved only...

18 January 2021 9,270 3 View

How to desinfect a liquid nitrogen tank?

Hey everyone, we'd like to clean and decontaminate our liquid nitrogen tank before stroreing new cells inside. The tank seems to be made of aluminum. Any recommendations on what disinfectant to...

07 January 2021 1,599 3 View

DIANA FEA 10.2: How do I model deformation supports, so that no tension occurs/is allowed ?

Hello together, I am working on concrete direct shear Tests, where i apply loadsteps of a prescribed Deformation (left deformation). Since i can only apply a Deformation with a support inplace i...

20 January 2020 8,225 3 View

Is HSE06 a good functional?

I'm investigating first row transition metal oxides on diamond surface, optimizing structures and calculating electronic properties (e.g. electron affinity). I was advised that since I'm working...

08 January 2020 8,713 4 View

Quick ('on the Fly') update of MM potentials, MD software with Amber ForceField?

Hello, Could anyone recommend a free MD software (that takes Amber ForceFields) where one can very quickly (on the Fly) update the MM potentials of a set of atoms (and query the coordinates of...

20 November 2019 7,716 1 View

What test shall I use when I want to compare an experimental group at various time points to one control group?

The data are as follows (overview of the design attached): One parameter was measured in the blood of the same patients at various time points and also in a healthy control group at one time point...

15 July 2019 1,382 6 View

Can I use ELISA with not ideal standard curve?

Dear colleagues, I would like to ask you if you have any experience how to use data from ELISA analysis when my standard curve is not as showed in the manual? I have many standard curves that have...

17 April 2019 282 4 View

Does it exist any open repositories of user stories?

For a Machine Learning-based project, I'm looking for repositories containing user stories (and acceptance criteria). This can be issue management systems (e.g., stories in Jira) or plain...

19 December 2018 4,195 7 View

What are possible portable multigas sensing devices?

Hi, I'm currently looking for a portable gas measurement system to measure O3, NO2 and CO at the same time. I already stumbled upon GrayWolf Sense and Aeroqual. Can someone suggest other...

13 September 2018 6,690 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View