Exist any method to quantify cytokines and chemokines in mouse brain tissue using any RNA multiplex assays?

More Fabian Lombillo's questions See All

¿Programas del gobierno de desarrollo economico agronegocios?

r los programas del gobierno acerca de los agronegociossabe

09 December 2023 6,402 1 View

Bibliography on Dentistry in Ancient Mesopotamia?

I am looking for Mesopotamian texts that discuss dentistry. I am searching for both secondary and primary sources. If you can help me, I would appreciate it.

26 October 2023 4,467 2 View

Any correlation between Pfefferkorn and Penetrometer for determine the viscosity of clays?

Hello all, do you know of a correlation between the upset height according to Pfefferkorn and the measured value of a penetrometer (in my case Penetrometer ST 207). The unit of the penetrometer is...

16 October 2023 7,655 0 View

Western Blot on primary human monocyte lysate doesn´t cause any bands?

Hope you´re all doing well! I´m currently in a bit of a situation. I have done Western Blot on primary human monocytes . I´ve tested the usability and approx. parameters used beforehand on THP-1...

10 September 2023 1,738 2 View

¿ que pasa con los nuevos conocimientos sobre la neuro educación y el tratamiento que podría tener con relación a los deportes colectivos ?

Me interesaría trabajar sobre las contribuciones de la Neuro educación a los deportes colectivos; Como influyen las emociones en el desarrollo de la educación física y e deporte.

17 August 2023 8,993 0 View

Contact cleaning of Perovskite Solar Cell?

Hello, the layout i use for making perovskite solar cells requires a lot of contact cleaning (5x5cm substrate). So far i used cotton swaps with DMF first and then cotton swaps with ACN. Problem...

22 June 2023 8,433 2 View

Serum free jurkat culture?

Has anyone ever tried to culture Jurkat cells in serum free media (+/- additional growth factors)? I'm looking for a way to reduce the amount of glycoproteins/lipids for prolonged culture. Best...

19 June 2023 457 6 View

Does anybody have the coordinates of the human IGH switch regions?

For a targeted sequencing project I would like to include the switch regions of the IGH locus targeted by the class switch recombination. The regions are not "officially" annotated for hg38, and I...

18 April 2023 5,493 5 View

Recovery of Lead Iodide for Perovksite Solar Cells?

I have a around 20g of PbI2 powder which was exposed to the atmosphere for long time. Now it is not possible to get a clear solution with DMF:DMSO. I assume it is due to moisture in the PbI2...

07 February 2023 7,611 1 View

Any one knows any paper that covers an analysis that covered both lower order and higher construct in PLS-SEM?

Hi, I'm currently looking at aspects of belonging and identity in relation to engagement and satisfaction and I wonder if there's any paper out there that covered both lower order construct and...

01 February 2023 4,789 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Lisette Chávez-Rodríguez

Hello Fabian, maybe you can check RISH (RNA in situ hybridization) technique.

Adrián Hernández-Díazcouder

Hello Fabian, you should check a mRNA array of inflammatory genes. You can found in Qiagen website.

https://geneglobe.qiagen.com/us/product-groups/rt2-profiler-pcr-arrays