07 April 2016 4 293 Report

What is the meaning of ESP in ESP culture system II for Mycobacterium tuberculosis diagnosis

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How does fluent FWH solve acoustic data at a location where no mesh is present?

In the simulation tutorial of the aeroacoustics around a cylinder by ANSYS, recievers are placed at a location where mesh isn't present, how does the solver predict the acoustics at that location...

16 October 2020 3,368 5 View

Problem with Cryosections?

Hi, I have a problem with cryosections while staining. Positively charged slides are used for cryosections, in spite of that during the staining process, the sections are washed away during the...

10 September 2020 3,767 3 View

Can we use 2, 6-dichlorophenol indophenol (DCPIP) method for analyzing crude oil degradation containing high sulfur content in the crude oil?

I was trying to find out whether my bacteria can degrade crude oil or not using DCPIP method that changes color after oxidizing hydrocarbons. But, I happen to order the crude oil containing 5%...

25 June 2020 9,801 2 View

Why my lenticrisprv2 plasmid after ligation of oligos is of smaller size than the expected?

after miniprep, I sequenced the plasmid. After sequencing I found that a region of plasmid was missing and its size if of around 8kb while it should be of around 12kb. It has happened to me for...

21 March 2020 736 3 View

HnE Protocol for the frozen section of cells encapsulated in hydrogels?

Hi all, I'm facing problems in staining the cryosection of the cells encapsulated in hydrogels. I'm using both charged slides as well as the albumin coated slides for the HnE staining. During the...

28 May 2019 1,197 5 View

VZV plaque assays?

Has anyone done plaque assays for VZV using toluidine stain?

10 March 2019 7,657 1 View

Has anyone done co-transfection with siRNA and the fucci system viral vectors by Thermofisher (Cat: P36238) ?

I'm trying to knockdown my protein in adherent cells and label cells with the fucci system for cell-cycle segmentation using microscopy. My protocol is as follows Day 0 : siRNA...

07 August 2018 4,362 4 View

Can anyone suggest me some ph.d research topic from machine learning and deep learning?

I am currently pursuing Ph.d

01 January 2018 7,222 17 View

What is the procedure for performing ELISA on protein pellet extracted from cell lysate?

Hi all, I wanted to perform ELISA (sandwich elisa) on the protein pellet extracted from cell lysate. I would like to know in which buffer, the protein pellet should be resuspended? Thank you Nisha

10 December 2017 9,325 3 View

Acceleration time history for Korba earthquake in India?

Can I get it in matlab format or notepad

08 October 2017 7,788 3 View

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