Erythropoietin receptor antibody to try on cultured cells - Astrocytes, neurons and microglia?

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38 kDa (maybe 31kDa too) band with ExoQuick-extracted exosomes?

Hi all, I've been using the Exo-Quick reagent by SBI for extracting exosomes from cultured medium from MSCs. The MSCs are cultured in exosome-free FBS for 3D prior to exosome extraction. The...

03 April 2019 6,693 0 View

How can I normalize to control when control value is 0?

When normalizing to control I usually divide the treatment reading with the reading for the control. But how do we deal with this when the control value is 0? For now, I'm just plotting absolute...

31 December 2015 7,497 7 View

Would H2O2 degrade in cell culture medium when put through a freeze-thaw cycle?

I'm trying to do an experiment where I injure neurons with H2O2 and apply that injured neuron medium to glial cells. For this purpose, I'll injure neurons for 30min and then freeze the medium...

06 July 2015 4,028 3 View

How to correlate protein expression to cell death on western blots?

I'm looking at the expression of certain proteins in control and injured brain astrocytes and neurons (isolated with MACS). I can't seem to find differences in expression when I know I should at...

09 October 2014 1,043 3 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

How to activate 4E8 T cells with plate-bound anti-CD3 for a genome-wide CRISPR screen?

It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...

17 July 2024 3,371 0 View

Questions of antibodies selection?

Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...

15 July 2024 6,008 5 View

Carsten Schmidt

Hi Praneeti,

are you still searching an anti-EPOR antibody for WB? Our company has recently manufacture one;

http://www.sysy.com/products/epo-r/facts-358103.php

which you could test. Please contact me, if you are interested.

Best,

Carsten

Praneeti Pathipati

Thanks Carsten, but we have finally settled on one that works for us. I appreciate you reaching out though!

no problem, if the antibody works, its fine. One of the golden rules I learned in the lab, nerver change a running clone.

Best regards,