Praneeti Pathipati

5 Questions 15 Answers 0 Followers

Questions related from Praneeti Pathipati

38 kDa (maybe 31kDa too) band with ExoQuick-extracted exosomes?

Hi all, I've been using the Exo-Quick reagent by SBI for extracting exosomes from cultured medium from MSCs. The MSCs are cultured in exosome-free FBS for 3D prior to exosome extraction. The...

04 April 2019 6,674 0 View

How can I normalize to control when control value is 0?

When normalizing to control I usually divide the treatment reading with the reading for the control. But how do we deal with this when the control value is 0? For now, I'm just plotting absolute...

01 January 2016 7,474 7 View

Would H2O2 degrade in cell culture medium when put through a freeze-thaw cycle?

I'm trying to do an experiment where I injure neurons with H2O2 and apply that injured neuron medium to glial cells. For this purpose, I'll injure neurons for 30min and then freeze the medium...

07 July 2015 4,005 3 View

How to correlate protein expression to cell death on western blots?

I'm looking at the expression of certain proteins in control and injured brain astrocytes and neurons (isolated with MACS). I can't seem to find differences in expression when I know I should at...

10 October 2014 1,018 3 View

Erythropoietin receptor antibody to try on cultured cells - Astrocytes, neurons and microglia?

We're trying to find a reliable EPOR antibody that we can use for western blots on astrocyte/neuron/microglia cell protein. Any recommendations?

08 August 2013 8,747 3 View