METHOD: What method to use for transient gene silencing in Cucumis sativus ?

I'm planning to silence candidate genes in the root system of C. sativus and viral vectors seem scarce in Cucurbitaceae and with low efficacy. Therefore, I'm looking for people who have experience...

06 December 2020 1,716 1 View

How to define receptor atoms in MOE?

How can a set/define atoms as being part of the receptor for docking in MOE? I have a covalently bonded ligand-receptor complex, and I would like to perform a docking experiment with the complex...

18 October 2020 9,688 2 View

Is someone used a Single or double point method for protein intrinsic viscosity determination?

Dear all, I am wondering if someone uses a single point or double point method to determine the intrinsic viscosity of their proteins. These methods are usually developed for polymers and does...

06 August 2020 6,227 5 View

Dynamic light scattering instruments for several conditions in parallel?

Hello, Our old but not to old DynaPro Plate reader I does not work anymore and Wyatt does not want to investigate the problem as the instrument is 10 years old. We have the money to pay them but...

14 December 2019 6,735 3 View

Why DHB is a reducing agent while sDHB matrix is not a reducing agent?

Hello everyone, My question is the next : Why the DHB matrix is a reducing agent while sDHB matrix is not a reducing agent? In this article it is written that sDHB is a non reducing agent Fast...

19 October 2017 190 3 View

What is the the most validated measure of Sluggish cognitive tempo (SCT)/Concentration deficit disorder (CDD) for 6-12 YO?

What is the the most validated measure of Sluggish cognitive tempo (SCT)/Concentration deficit disorder (CDD) for 6-12 YO? Ideally a questionnaire with a parent and teacher report version

08 February 2017 6,163 1 View

Why do cells round up upon DCFDA (ROS-staining)?

25 November 2014 4,325 6 View

Do you know any good french corpora for named entity detection?

I'm looking for a french corpus tagged with named entities in order to train supervised machine learning. Can you help me to find one?

06 March 2014 7,268 3 View

How can I get the specific RNA expression of an alternative transcript of a gene of interest?

04 November 2013 9,908 6 View

What tools do you use for illustrating your articles?

Each time I write an article, I have to struggle with the problem of the illustrations. I can often see pretty nice figures in articles, more especially in reviews, which makes me think that my...

02 June 2013 3,473 20 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

How to find natural frequency of a bridge from vehicle induced vibrations?

i have frequencies of bridge that are effected by mass of vehicle. the problem is when any vehicle pass the bridge it would add the its mass to the bridge mass ultimately mass of the bridge will...

22 February 2021 10,004 3 View