Hello to all researchers
I would like that someone can guide me if I can validate my results.
I tested the activity of a positive clone which produces a lipase (metagenomic DNA) directly in the fosmide pcc2, for your information the recombinant clone shows activity only at high temperature, so i start a culture of the positive hit in LB + CAM + Induction solution to induce number copy of pcc2f.
I managed to obtain a curve defining the optimum activity (80 ° C), after having done 5 repetitions, the problem is that the activity (the absorbance is low) 0.1457.is can I validate these results or I have to clone the gene in question for an overproduction.
thank you
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