I would bé gratful if you could send me a protocol to extracted metagenomic Dna from soil. The aime of work is shotgun metagenomics sequencing,so i'm looking for a good yield ans concentration. I already try with the power max kit of quiagen comptant and the concentration is very low.
Thanks
"Comparative studies on the extraction of metagenomic DNA from various soil and sediment samples of Jammu and Kashmir region in prospect for novel biocatalysts" by R singh.
This is perhaps the best protocol that have yielded me excellent results.
The methods of Zhou et al. (1996), Volossiouk et al. (1995), Tsai and Olson (1991), Siddhapura et al. (2010), Verma and Satyanarayana (2011) are very helpful too.
In case you want to use a kit for soil DNA isolation try the FastDNA™ SPIN Kit for Soil by MP Biomedical. It works for most of the samples types. I am also struggling to isolate DNA from saline soil having tried most of the manual methods as well as kits.
Yes,metagenomics extraction of DNA is the best. But when you are experiencing low level of DNA,then check humus content,it may be a hindrance! Also,try changing the extraction kit. Use bioline product, it will help in getting more concentration of DNA.
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