Does anyone have experience with the isolation and cultivation of enterocytes?
Thanks
I searched DNA motif in whole genome using FIMO and now I would like to add gene names into the table with founded regions containing my DNA motif. Can anyone help?
10 February 2023 9,069 1 View
Is there a fluoride home application compliance test, that would tell the use of fluoride in past week or month? Something similar to the glycated hemoglobin test that tells you your average...
28 November 2020 229 5 View
Hey guys, I'm looking for advise. We want to measure stress level during task in virtual reality. We are using simple heart rate monitoring, unfortunately not to accurate but sufficient. But we...
19 September 2019 8,160 1 View
Excluding pupilometry, is there any method, how to measure immedate stress response? First I was thinking about hearth rate than about skin conductivity. In both cases there is to long delay...
05 September 2018 9,695 3 View
We seek to determine reward decision making, outcome evaluations, error signalling and adjustment of response in rats, what is the best way (T-maze, operant tasks, AST)? Thanks for any...
24 May 2018 3,986 4 View
Is it better to use methanol-free formaldehyde for DNA-protein crosslink?
13 November 2017 3,886 4 View
Hi, could you give me an advice about chromatin immunoprecipitation in bacteria? Is it possible to use DNase for removal nonbinding part of DNA? I mean this: protein will be bind on part of...
26 September 2017 6,906 2 View
I am looking for some protein tags for 9 kDa protein in gram-negative bacteria. Do you mind that Tag-ChIP-IT® is appropriate for bacterial ChIP or woudn´t it be better to use FLAG or 3xFLAG and...
23 May 2016 987 1 View
I'm dealing with Upper Cretaceous microfossil association that consists of ostracods and foraminifera. Source material – sandstone and marly limestone – was disintegrated in water with some...
24 August 2015 2,611 33 View
There have been long discussions on RNAlater composition, how to make a home-made RNAlater etc. but I'm wondering - and I would love to hear especially from those scientists who actually tried to...
23 June 2015 2,964 3 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
What did the plantation economy in the South produced and all the crops cultivated in north India cannot cultivated in south India why?
26 June 2024 7,264 0 View
I would like to become self disciplined and work by myself without supervision. How do I achieve it when I am not interested at all in working without any push by others?
24 June 2024 4,529 5 View
Usually, basal media have been recommended for the culturing of human epidermal keratinocytes, and most articles suggest growing them in serum-free media. I'm finding it a bit challenging to...
24 June 2024 2,609 3 View
One of the major objectives of Natural Farming is self-reliant farmer and promotion of traditional varieties so that the farmers can use their own seeds. But it is observed that most of the...
11 June 2024 5,696 0 View
Does dry weather limit tillage options for timing cultivation to complement conservation plans?
02 June 2024 1,735 5 View
What specific challenges do heat waves pose to vegetable cultivation in India?
28 May 2024 5,155 1 View
Hello. I have cultivated the species of dermatophytes in the special culture medium of SCC. Unfortunately, the rate of species growth is very slow. Does anyone have any experience in this field,...
26 May 2024 4,552 3 View
Hi! I left some petri dishes with Pseudomonas aeruginosa cultivated in MHI in the fridge (8°C) during some time off, and when I came back, it was like this. This is just one plate out of many, and...
24 May 2024 5,149 3 View
For a set of experiments, we need to use insulinoma NIT-1 cells (ATCC CRL-2055). First time we get them from ATCC, none of the cell attached to the flask. We just received a second vial of cells,...
21 May 2024 9,390 0 View