Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to Interpret Partial Eta Squared Numbers For a Repeated Measures Anova?

I have obtained Partial Eta Squared Numbers through Two-Way Mixed Anova Analysis. My results for different constructs are: .105, .135, .038, .068, .061 respectively. I wanted to know, whether...

24 February 2021 7,122 1 View

Please. Could you help be in implementing Fractal Discrete Cosine Transform algorithm in Matlab?

I do need the Matlab code of Fractal Discrete Cosine Transform (FDCT). Can anyone who has already implement this code, help me with the implementation of this transform?

24 February 2021 5,602 2 View

Can we proceed to perform Two-way Mixed-ANOVA despite normality violations?

While testing assumptions, Shapiro-Wilk Test failed. However, I proceeded with the analysis and the interaction effect was significant. During our course- lectures, it was mentioned that Anova...

24 February 2021 228 3 View

Problem in energy minimization using Gromacs?

I have done energy minimization of DNA using Gromacs with tutorial lysozyme in water. The potential energy curve show a steep drop.

18 February 2021 4,822 2 View

What is the reason film thickness increased?

what is the reason film thickness increased when increased doping concentrations give some reasons The measured film thickness is 156, 282, 394, 548 and 681 nm for 0, 1.5, 2.5, 3.5, and 4.5 wt....

17 February 2021 8,987 5 View

What is the best method for preparation of Polymeric nanoparticles for co-delivery?

We are planning to make Polymeric nanoparticles for co-delivery of 2 flavanoides. Which is the best method, that one should follow.

09 February 2021 7,086 8 View

Queries regarding the rain flow counting algorithm?

02 February 2021 9,790 2 View

Is there any software to generate pdb of G quadruplex?

I have a DNA sequence which has predictability to form G quadruplex . I need a software to generate a pdb of it which will form G quadruplex. I am using open Babel but I am not able to set the...

29 January 2021 6,190 4 View

How to determine the genetic potential of the genotype in terms of fruit size and weight?

In addition to genetic and environmental factors, fruit size is also influenced by crop load, rootstock, orchard planting system/tree training system, pollination, water and nutrient management,...

13 January 2021 9,309 4 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

Online surveys for social sciences researchs: what are your opinions about this tool and how can we improve the research design to incorporate online?

Good morning everyone, Due to the pandemic, I conducted several online surveys. It is quite agile, not at all expensive, but there are several problems: the sample's representativeness and its...

21 February 2021 7,457 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

Hi, can a variable in a questionnaire be measured with a series of questions or it has to one question for one variable?

I am doing research on the impact of competitive bidding on project success and would like to use SEM. Project success shall be: cost overage, completion time, quality of works and variation...

13 February 2021 4,413 6 View

Can you realize "top-down" and "bottom-up" ARE THE SAME THINGS at important junctures IN ONTOGENY (child development)?

Can you realize "top-down" and "bottom-up" ARE [ or certainly can, if not MUST, be ] THE SAME THINGS at important junctures IN ONTOGENY (child development)? This Question is NOT addressing YOU...

09 February 2021 5,289 1 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

What is your opinion regarding opening of school? Now students are supposed to join school. Do you think that decision is in the interest of society ?

From long Pendamic now government is opening school and colleges on for off line classes. What do you think that this decision is fruitful to the students and society too?

08 February 2021 3,068 6 View

Fractional derivative users, are you on the same page with me on the validity of the fractional order parameter, say alpha?

Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...

08 February 2021 9,671 3 View

Looking for a scale to measure attitudes towards violence against women and the factors that impact attitudes in South Africa?

I am interested in doing research on attitudes towards violence against women and the factors that impact attitudes in South Africa? Does anyone know which attitude scale I can use to measure...

06 February 2021 3,076 1 View