How to remove the species data from rda data frame in R (only remain the sites/samples point)?

Dear everyone, When I using the rda function in r, I met this question. As you can see in the figure, there are a lot of deep blue points which refers to the species. But I would like remain...

15 February 2021 8,982 2 View

Whether L version and D version peptidesshould have identical NMR, especially in the state of self-assembly?

Whether L version and D version peptides should have identical NMR, especially in the state of self-assembly have to have identical NMR? How to use HRMS to confirm L version or D version...

01 February 2021 6,756 4 View

What are possible factors influencing the isotope-based hydrograph separation in river confluences?

When performing hydrograph separation (EMMA method) with isotope tracers in river confluences (natural large basin), we found that the contribution of each end member sometimes exceeds the...

20 January 2021 8,403 5 View

Can you have pressure inlet and velocity inlet at the same time?

I was reading a reference (Reitz-A Photographic Study of Flash Boiling Atomization). In his experiment, his boundary condition is delta p and constant velocity. The liquid injection pressure was...

18 January 2021 842 9 View

How to determine additional cycle numbers using qPCR in ATAC-Seq?

I am doing ATAC-Seq with OMNI-ATAC-Seq protocol (Kaestner Lab 2019). I did PCR amplification for 5 cycles of my library. Now I use 5ul of the PCR product to do a qPCR to determine how many...

12 November 2020 3,678 1 View

How to visualize influenza PR8 plaques while the infection is successful?

Hi, everyone, I am optimizing my protocol of influenza PR8 plaque assay. I am following the classic protocol. But I ran into problems when I am trying to visualize plaques at the high virus...

25 August 2020 2,017 1 View

How to extract absolute refractive indices of an optical fiber from an refractive index difference plot?

10 May 2020 2,402 13 View

Do you think current guidance for COVID-19 prevention is correct?

CDC provided guidance to the public "Wash hand, disinfect surfaces, cover sneezing" ever since the outbreak of COVID-19. I get a strong sense this guidance is for contact transmitted disease...

01 April 2020 8,279 1 View

Where is the TSS (transcription start site) following CAG promoter?

The commonly used CAG promoter is usually labeled as three parts, take pCAGGS-mCherry (https://www.addgene.org/41583/) for example. CMV enhancer + chicken beta-actin promoter + chimeric...

13 February 2020 7,125 2 View

Google scholar inflates citation count?

Google scholar included under "Cited by" a few papers that did NOT cite my papers or book chapters. This inflates citation counts of some of my publications. How can this be corrected?

29 November 2019 2,469 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View