We bought the Ki67 (27309-1-AP, proteintech, Cited Reactivity Human, Hamster, Pig). But we are not sure if it is suitable for us to use this primary antibody to detect Ki67 in mouse liver sample (IHC, Immunohistochemistry)?
Because the Cited Reactivity is Hamster but not mouse. But we found some papers (mouse sample IHC) cited this antibody on the webpage ( https://www.ptgcn.com/products/KI67-Antibody-27309-1-AP.htm) So we are so puzzled.
Could you please give us your precious research experience on this issue?
Ki67 immunohistochemistry is a widely used marker of the tumor proliferative fraction. Apart from the nuclei staining of dividing cells, MIB-1 monoclonal antibody was withal found to stain the cell membrane of some tumour types. Indeed, such membrane reactivity was proposed as a diagnostic feature of the hyalinizing trabecular tumour (HTT) of the thyroid. To verify the diagnostic role of the Ki67 membrane pattern, 6 HTTs, 8 pulmonary sclerosing hemangiomas (SH), and 6 other human tumors with MIB-1 cell membrane immunoreactivity were stained by immunoperoxidase with 5 different anti-Ki67 antibodies in different experimental conditions. We show here that the cell membrane reactivity reported in HTT is engendered only by MIB-1 and not by other antibodies to Ki67 (including commercially available mouse and rabbit monoclonal antibodies). In integration, this particular pattern is obtained only if the reaction is performed at room temperature because automated immunostained which operates at 37 degrees C does not engender any MIB-1 membrane localization. The same findings were obtained in the other 6 tumors. Conversely, sclerosing hemangioma of the lung did not engender any MIB-1 cell membrane reactivity in our hands. Cross-reactivity of the MIB-1 monoclonal antibody with an epitope expressed at the cell membrane level (rather than an artefact) seems the most likely explication for this finding because the immunoreactivity is generally profound and uniform in the membrane-positive tumours. We conclude that when Ki67 immunohistochemistry is utilized for diagnostic purposes in a suspected HTT, only the MIB-1 clone at room temperature should be employed. Shuang Xia @Lord James Henderson Mitchell https://dailyplanet.club/
Dear Lord James Henderson Mitchell, thanks for your kind reply. We will try this experiment.
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