We are interested in generating sections from live embryonic tissue for subsequent imaging and culture. We work with the early chick embryo and sub dissected tissues, ideally we would like to section between 100-200 microns in thickness on a vibratome. Most protocols use low melting agarose and we have had ok success with that. I wished to see if there were alternative embedding approaches, perhaps gelatin or other substrates. I welcome any suggestions or protocols.