Electroporation protocol for BHK cells?

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Vedita Singh

Thanks Ted for your reply.

As of now we have electroporation facility in our lab. So if you have suggestion for the same then please share.

In future I will also try lipofection technique as well.

Jasmine Madrigal

I've used Exponential setting to electroporate viral RNA into BHK21 cells with some success.

Settings: 850V, Capacitance 25, and Resistance infinite

Adarsh K. Gupta

2 pulses of 460V, 725 ohm, 75 uF using BTX ECM 630 Electroporation System. Works well.