Vedita Singh

4 Questions 11 Answers 0 Followers

Questions related from Vedita Singh

Electroporation protocol for BHK cells?

Hello, I am planning to electroporate viral RNA in BHK cells, with maximum efficiency. Please share the electroporation protocol for BHK cells and and suggestions if possible. Thanks and...

25 July 2018 8,421 3 View

Is there be any possibility that the more than 300 nucleotide region of coding gene and 3' UTR can overlapp ?

The genome I am looking at is an arboviral genome, which is around 12 Kb long. There is a mismatch in number of nucleotides (which I obtained from NCBI) in 3' UTR region which is next to coding...

01 December 2016 4,054 0 View

Will lymphocytes be able to attach to the fibronectin coated culture flask ?

Dear all, I need answers with references. (I want to culture adherent cells of lymphocytes. Will this strategy work ? )

19 September 2016 3,630 3 View

Which one is more efficient for cell disruption and protein purification , a constant cell disruptor system or a cell sonicator?

Here the cells used are E.coli DH5alpha.

23 August 2016 5,504 9 View