Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

Reliable and Valid Instrument for Causes of Medical Errors?

Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!

15 February 2021 2,874 4 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

Software for flow cytometry-like gating of data sets?

I am currently trying to do image based cytometry from fluorescence microscopy images. From the images I extract data such as cell size and a number of fluorescence intensities. Is there any...

09 February 2021 6,278 1 View

Can A. thaliana splice algae genes?

24 January 2021 329 2 View

Does long term preservation (10years) of Collembola in 99% Ethanol affect recovery rate of extraction by flotation?

Hello! I am currently developing an automated counting approach using Fiji to count Collembola from pitfall trapped samples that have been stored in Ethanol (99%) since 2010. I use flotation (tap...

13 January 2021 659 2 View

Co-stain Lectin PNA FITC conjugate with an uncojugated primary antibody?

Hello, I am planning to stain retinal tissue sections for IHC and want to co-stain cones and rods. For this we plan on using a Lectin PNA From Arachis hypogaea (peanut), FITC Conjugate for cones...

12 January 2021 583 1 View

How to use Abaqus USDFLD subroutine to vary stiffness?

Hi I have a problem where I am trying to vary the stiffness spatially across a structured interface. The whole idea is to vary the stiffness vertically, with a bulk stiffness of 8GPa decreasing...

06 January 2021 262 3 View

I really need help with cov-2 pseudotyped lentivirus production. Support?

Hi, i'm desesperate. i'm trying to produce lentivirus usinf BEI resources kit NR-52948. 24 hours after transfection i detect a massive GFP expression in my 293T cells. After 72 hours i filtered...

30 December 2020 9,889 6 View

How can i estimate the total phenolic content from a plant extract?

Suggestions and recommendations are welcome. A protocol used and experience would also be helpful.

25 December 2020 9,807 4 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Are cloud points able to identify tree species?

Hello Dears My goal is to classify dense forest tree species using drone images. Is it possible to classify dense cloud points? If yes, in what way and in what software? Thankful

15 February 2021 3,475 3 View

Has anyone used Whatman FTA Plant Saver cards for collection of fungal DNA?

I would like to barcode fungal caps from the field for two main research goals (1) molecular iD of the fungal specimen and (2) to identify any invertebrate DNA within the cap using CO1...

08 February 2021 4,486 1 View

To detect Diazepam per HPLC in less that 10 min . How ?

We Get the Problem in the University laboratory that we detect Diazepam Late. Our goal is to try to detect it in less than 10 min We wrote to the company and it suggested the method below (The...

14 December 2020 1,265 3 View

Are open source PCR primers and cellular reagents of value to locating COVID infections on the global map?

A concern with commercial COVID-19 diagnostic test kits is that they tend to be expensive, and may be difficult to access in places far away from the companies that produce them. In order to...

10 December 2020 5,965 2 View

How do you create a funnel plot in STATA to assess publication bias using proportions from the metaprop command (for a meta-analysis)??

Hi everyone! I am trying to use STATA to create a funnel plot to asses publication bias using proportions from studies for a large meta-analysis (having used the metaprop command). I"m having...

09 December 2020 6,096 3 View

What is the best flight altitude for a drone in the forest?

My goal is to use the short-range remote sensing technique (drone) to study tree species in deciduous forests. Overlap and flight altitude play a key role in this. Do you have the right overlap...

02 December 2020 2,442 4 View

How to define acceptable log10 range for real time pcr quantitative assay?

Case: I have performed inter laboratory comparison for 2 samples and obtained our lab and referral lab value in IU/ml. As per NABL guidelines (India) the acceptable range between the two values...

25 November 2020 4,112 1 View

How can we test interaction effect using repeated measure design?

Hi! I am currently working on a problem using repeated measure design. Each participant was assigned to two CONDITIONs (A and B). And their GOAL (continuous) were measured twice in condition A and...

25 November 2020 1,235 3 View

To become an Ethologist, what classes should I take freshman year of college to set me on that path?

I am currently a freshman in college aiming to become an Ethologist. I am wondering what classes I should take freshman year to set me on that path. My ultimate goal is to study big cats (lions,...

15 November 2020 5,212 15 View