EGTA vs BAPTA? - FAQS.TIPS

14 April 2017 1 7K Report

How to choose between EGTA and BAPTA for extracellular calcium chelation? Both of them have similar Kd values but from what I read, EGTA has a much slower Kon rate compared to BAPTA which has a faster rate of binding. So can you give me examples of instances as to where you will use EGTA and where to use BAPTA

Sanath Kumar Janaka

As I understand it, BAPTA is a lot more specific for calcium thaan EGTA. If you need specificity of calcium ion chelation, I would choose BAPTA. Especially if you use it in cell culture, pH sensitivity of Ca binding by BAPTA is minimal.

Badges
Science topic

More Ishwarya Murali's questions See All

How to calculate mmpbsa of a metal containing protein -DNA complex?

I am calculating its MM/PBSA of protein DNA complex having magnisium ion as a part of receptor in which I got this error every time

19 May 2024 3,361 0 View

Extending Conference paper to a journal paper?

If a journal and conference belong to the same entity(like EAI or ACM), and the journal has specifically asked you to give them an extended version of the conference paper, do we need to be...

15 February 2024 2,008 2 View

In the calculation of MM-PBSA by Amber tools, for a protein -DNA complex I found the error,how I resolve this?

I have completed MD simulations of protein -DNA complex upto 200ns using Amber tools but when I am calculating its MM-PBSA I have the same error every time

10 February 2024 2,623 2 View

What is the work function (in eV) for a silver nanowires?

The work function of bulk silver is cited as 4.6eV. Will there be any change in the work function of the metal when the dimension is reduced to nanometer? In this case a nanowire whose diameter is...

21 September 2023 7,529 3 View

In equilibration and production output there are some like this"ATTEMPTING M.C BAROSTAT CHANGE: failed".What is the logic beyond it?

In A protein -DNA complex I have completed minimisation heating upto 300 and Density but the error give in case of equilibration and production

01 July 2023 6,954 0 View

Formal verification of design?

formal verification papers

25 May 2023 3,846 2 View

I Want a plagiarism software for my personal use , please share where it available (Link) , price detais?

My own question

15 March 2023 6,905 7 View

What is the best suited method to evenly distribute rGO on a glass subtrate?

The substrate used is a microscopic glass slide. rGO is dispersed in NMP (N-Methyl-2-pyrrolidone) to form a dispersed solution. What is the best suited deposition technique to obtain an even...

05 March 2023 5,599 2 View

Is it possible to produce thin flakes of rGO?

rGO was obtained by reducing GO that was synthesized by modified hummers method. If it is possible to produce thin flakes of rGO from a bult rGO, how could we characterize so that I can prove the...

28 February 2023 5,609 4 View

What is the difference between transverse and longitudinal plasmonic resonance?

Does transverse and longitudinal plasmons fall under localized surface plasmons? What is the significant difference between them? At what level will this affect the fabricated silver nanoparticle...

15 January 2023 5,699 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under op microscop?

How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?

07 August 2024 2,132 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds?

XRD Analysis is showing only Calcium carbonate. It is not showing other compounds. Can anyone help me get the other compounds

04 August 2024 3,019 3 View

Why don't d-orbitals split themselves, why does it take a ligand? why don't protons from ligand repel nucleus split d-orbitals?

why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...

03 August 2024 3,589 3 View

Why doesn't chromium 2+ ion use all its d-orbitals to receive lone pairs from six waters in [Cr(H20)6)]3+?

I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...

03 August 2024 1,370 1 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Looking for help on sem image analysis?

Hello I am conducting a microstructural analysis of a soil treated with lime. The following sem images are of the untreated s1 and treated soil s3. The untreated soil contains quartz calcite...

01 August 2024 572 0 View

Is artifacts in XPS possible to build high deviation in binding energy larger than 5 eV??

Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...

30 July 2024 9,824 2 View