Hello, I am planning to do a research about the effect of salinity on the germination and growth, there are several things that still confuse me which are :
1. Should the germination be done in a dark place?
2. Would you kindly suggest a simple protocol to determine the concentration of proline in plant tissue?
3. How does the salinity stress affect the leaves ?
4. Will it be okay if the medium is composed by Perlite : Cocopeat (1:1) ?
Dear Grace Helena
- Wheat-grass seeds can well germinate in darkness.
- Salinity reduces leaf area, water content, chlorophyll content and photo-chemical efficiency of photo-system II, enhances leaf temperature and proline content, and also causes membrane damage and ion imbalance through primary or secondary stresses, depending on salt level, species and genotype.
- you can use perlite : cocopeat (3:1) as medium.
Regards
Kazem Ghassemi-Golezani
You can also measure antioxidant enzymes like SOD, CAT, GPX, APX and MDA. Then you find the ratio of antioxidants to MDA among your genotypes.Then you find the correlation between seed yield and your finding ratio with statistical methods.It will show you resistant genotypes.
Good luck.
I'm confirming the top suggestions from Dr. Ghassemi-Golezan, Hesam Shahrajabian and Habibi. They have a good experience in that case.
The best method to calculate proline content is by Bates et al. 1973, steps are simple and well described by Hesam Shahrajabian sir. You can also go for anti oxidant enzymes activity which will give better understanding on effect of salt stress. You can also use hydroponic medium instead of medium is composed by Perlite : Cocopeat.
Certainly Wheatgrass seed need darkness to germination. Salinity reduce plant growth and increase damage in morphological traits. I recommend to read this article for more details.
Article Osmolyte Accumulation and Sodium Compartmentation Has a Key ...
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